详细描述:
Phospho-PKCδ (Thr505)抗体识别内源性的Thr505磷酸化的PKCδ蛋白。此抗体与其它磷酸化的PKC亚型α, β, γ, ζ或ε没有交叉反应。该多克隆抗体用与小鼠PKCδ中Thr505位点附近的氨基酸序列对应的人工合成磷酸化的肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。Phospho-PKCδ (Thr505) Antibody detects endogenous levels of PKCδ only when phosphorylated at Thr505. This antibody does not cross-react with the phosphorylated forms of PKC isoforms α, β, γ, ζ or ε.Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr505 of mouse PKCδ. Antibodies are purified by protein A and peptide affinity chromatography.