详细描述:
Phospho-4E-BP1 (Ser65) Antibody检测仅在Ser65位点磷酸化的内源性4E-BP1蛋白水平水平。该抗体也能够识别在丝氨酸101磷酸化的4E-BP1蛋白(参见应用文献, Wang et al. 2003)。通过人工合成小鼠4E-BP1 蛋白相应序列的多肽片段免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Phospho-4E-BP1 (Ser65) Antibody detects endogenous levels of 4E-BP1 when phosphorylated at serine 65. This antibody has been shown to also recognize 4E-BP1 when phosphorylated at serine 101 (See application reference, Wang et al. 2003).Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence of mouse 4E-BP1. Antibodies are purified by protein A and peptide affinity chromatography.