详细描述:
该抗体能够特异性检测内源性在Asp353处裂解的鼠源caspase-9 大片段(37 kDa)。该抗体与全长的caspase-9或其他caspases不发生交叉反应。该多克隆抗体是采用合成的鼠源caspase-9 Asp353位点周围氨基酸序列相对应的多肽免疫动物后制备获得。抗体通过蛋白A和肽段亲和层析而纯化。Cleaved Caspase-9 (Asp353) Antibody (Mouse Specific) detects endogenous levels of the 37kDa subunit of mouse caspase-9 only after after cleavage at aspartic acid 353. It does not cross-react with full length caspase-9 or with other caspases at endogenous levels.Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp353 of mouse caspase-9. Antibodies are purified by protein A and peptide affinity chromatography.