详细描述:
DNP(2,4,二硝基苯)半抗原偶联羧甲基氨基乙酯-聚蔗糖(AminoEthylCarboxyMethyl-FICOLL , AECM-FICOLL),是一个高分子量的多糖。半抗原-聚蔗糖偶联物作为一类T不依赖型抗原,用于研究B淋巴细胞活化。
DNP (2,4,Dinitrophenyl) hapten is conjugated to AminoEthylCarboxyMethyl-FICOLL (AECM-FICOLL), a high molecular weight polysaccharide. Hapten-FICOLL conjugates are useful as T-independent antigens for studying B lymphocyte activation.(1)
属性:
化学名:2,4,二硝基苯-羧甲基氨基乙酯-聚蔗糖;
形态:黄色粉末;
最大吸收光(Lambda Max):360;
Lambda Max下的消光系数:17400;
产品用法:
该产品可在PBS缓冲液、水或其它1 mg/mL的中性缓冲液中溶解。溶液储存时若出现沉淀,使用前简单离心,仅使用上清即可。溶解后分装-20°C避光保存。偶联物利用1.74 x 10E+04作为DNP的消光系数在360 nm下检测,DNP比ACEM Ficoll的摩尔比率以假设ACEM Ficoll的分子量为400,000为前提给出。;
保存及运输:
运输条件:常温/蓝冰/干冰;
储存条件:常温;
Properties:
Chemical Name: 2,4,Dinitrophenyl-AminoEthylCarboxyMethyl-FICOLL;
Appearance: yellow powder ;
Absorption Maximum (Lambda Max): 360;
Extinction Coefficient at Lambda max: 17400;
Product Usage:
This product can be dissolved in PBS buffer, water or other neutral buffers at a concentration of 1mg/mL. Product may precipitate when stored in solution, therefore it is recommended to centrifuge the solutions briefly before use and use only the supernatant. Store solution in aliquots at -20°C. Protect from light. Conjugation is determined at 360 nm using 1.74 x 10E+04 as the extinction coefficient of DNP and is given as a molar ratio of DNP to ACEM Ficoll assuming the molecular weight of AECM Ficoll is 400,000.
Storage and Handling:
Shipping Conditions: Ambient;
Storage Conditions: Room Temperature