详细描述：
LCG Biosearch Micropure纯化柱通过进行柱上去三苯甲基化，用于反向纯化5'-DMT（5'-二甲氧基三苯甲基）保护的寡核苷酸。净化滤芯由填充有高性能疏水聚合树脂的柱子或注射筒组成。树脂能够强力结合5'-DMT保护的产物，从而将不结合的常见杂质和截短序列分离。随后用酸处理即可从产物中除去DMT基团。用20％乙腈洗脱所需产物（无有机残余物）。将DNA纯化柱与LGC Biosearch的Microsync ll真空歧管系统结合使用，可同时快速，高效地纯化大量样品。微纯化柱也可以用于寡核苷酸的简单脱盐。
BTI's MicroPure II columns (MP-1602) are intended for Reversed-phase purification of 5'-dimethoxytrityl protected oligonucleotides followed by on-column detritylation. The cartridge consists of a 5 mL syringe barrel packed with 200 mg of high performance hydrophobic polymeric resin. The method relies on strong binding between the 5'-DMT protected product and the resin, thus allowing separation from the most common impurities, truncated sequences, which do not bind. Treatment with acid removes the DMT group from the product, the desired product (free from organic residues) is eluted with 20% acetonitrile. The method is rapid, efficient and many samples can be purified simultaneously. At least 1 µmol or 50 O.D's of crude DNA can be applied to the column. The purified yield will depend, in each case, on sequence length and the quality of the crude sample.
