详细描述：
Oligonucleotides (whether synthetic or natural) may be purified by a number of techniques, including polyacrylamide gel electrophoresis and high performance liquid chromatography (either by ion exchange or reverse-phase methods). Synthetic DNA can be conveniently de-salted and purified by reverse-phase low-pressure separation on BTI's SuperPure (SP-1000) columns. BTI's SuperPure Columns are intended for reverse-phase purification of 5'-DMT oligonucleotides followed by on-column detritylation. The method relies on strong binding between the 5'-DMT protected product and a hydrophobic optimized polymer packing (polystyrene), thus allowing separation from truncated sequences which, due to a lack of DMT group, do not bind. Treatment with acid removes the DMT group from the product, the desired product (free from organic residues) is eluted with 20% acetonitrile. The method is rapid, efficient and permits many samples to be purified simultaneously. The SuperPure column has capacity to handle crude, cleaved DNA from a 50 nmol scale synthesis. The purified yield will depend, in each case, on sequence length and the quality of the crude sample.
