化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用,可用于PCR,基因组测序,SNP基因分型和基因合成等。
LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料,可用于合成复杂的qPCR探针。
生物素C6 T亚磷酰胺允许在寡核苷酸内部或5'末端标记生物素功能团,可以通过酶标记的抗生物素蛋白或链霉亲和素偶联物进行检测。该生物素亚酰胺基于胸腺嘧啶残基,标记时可按标准杂交参数如正常熔解温度进行操作。
The Biotin C6 T amidite allows for the incorporation of biotin functionality within an oligonucleotide or on the 5' terminus. Biotin labeling can be detected through the use of enzyme labeled avidin or strepavidin conjugates. This biotin amidite is based on a thymidine residue which when incorporated allows for standard hybridization characteristics such as normal melting temperature.