化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。
LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。
其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。
BHI-1亚磷酰胺用于标记荧光探针的5'端或内部。该亚酰胺含有DMT保护基，其按传统方法即可去除。BHQ-1染料在480至580nm范围内具有最大吸收峰，能够高效淬灭发射光谱在该范围内的荧光基团。
BHQ®-1 amidite is used for the 5' labeling of fluorogenic probes or to place the BHQ moiety internally. This amidite contains a DMT protecting group, which is removed under traditional conditions. BHQ-1 dye is designed to have maximal absorption in the 480 to 580 nm range, which provides excellent quenching of fluorophores emitting in this range.
