BHQ-0 Dark Quencher, abs 430-520 nm; 3' Modification
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详细描述:
LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
3-BHQ0修饰将非荧光的Black Hole Quencher 0染料添加到寡核苷酸的3'末端。 3'标记的寡核苷酸用于广泛的应用中,包括用于实时定量PCR的双标记荧光探针。BHQ-0黑暗淬灭剂具有从430nm至520nm的强吸收光谱,能够有效猝灭该范围内发射光的荧光基团。
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
The 3-BHQ0 modification adds a non-fluorescent Black Hole Quencher®-0 dye to the 3’ end of an oligonucleotide. 3' BHQ®-0 labeled oligonucleotides are used in a wide array of applications, including dual-labeled fluorogenic probes for real-time PCR. The BHQ-0 dark quencher has strong absorption from 430 nm to 520 nm, which provides excellent quenching of fluorophores that fluoresce in this range.