Base Charge + Purification, Primer, RP HPLC Purified
产品概述技术指标订购信息服务支持
详细描述：
无论您需要标记还是未标记的寡核苷酸或PCR引物，Biosearch Technologies都能满足您的确切需求，因为我们以极具竞争力的价格提供Oligo定制服务。大多数寡核苷酸的合成周期不到5至7个工作日，且可根据需求提供分析证书COA。
LGC Biosearch“构建您的定制寡核苷酸（Build Your Custom Oligos）”服务可定制DNA，RNA，甚至DNA/RNA杂交序列。如果您想订购双标记探针，请使用我们的双标记BHQ®探针快速订购表格。我们还提供其他荧光探针和引物修饰形式。
几个订购小贴士：
1. 如何定制带RNA碱基的寡核苷酸？
通过从Internal Modification（内部修饰）下拉菜单中选择rA，rC，rG或rU，如果您的序列全部是RNA，选中RNA复选框即可。
2. 如何定制具有内部修饰的寡核苷酸？
1）如果您的寡核苷酸需要内部修饰，请选择修饰类型，然后单击添加按钮，内部修饰就会插入到光标的位置。
2）添加由T（...）注释的内部修饰表示一个胸腺嘧啶连接在内部修饰上时，将在序列中引入另外的T碱基。
3. 如何定制具有不确定/降解碱基的寡核苷酸（什么是不确定碱基？请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles）
为了确保寡核苷酸能够被有效识别，当在序列中输入不确定/降解碱基时，请使用IUPAC代码，而不是括号如（A/G），（A/G/C/T）等。
4. 合成规模 vs.更终产量
由于探针/引物在合成过程中需要经过多个步骤，如每个碱基的偶联、寡核苷酸从固相支持物上的切割和纯化步骤，会导致合成规模和更终产量不同。经过这些步骤后，寡核苷酸的更终产率会有损失，将小于合成规模（起始材料）。
5. 订购在板中交付的寡核苷酸
需要订购大量寡核苷酸？请访问我们的高通量定制网页，您可以在其中下载我们的列或行格式excel订单。更少起订量48条。
Whether you require labeled, unlabeled oligos, or pcr primers, Biosearch Technologies can meet your exact needs as we offer custom modifications at extremely competitive rates. Most oligos are shipped in less than five to seven working days. Certificates of Analysis are available upon request.
The "Build Your Custom Oligos" Quick Order form allows you to build your custom DNA, RNA, and even DNA/RNA hybrid sequences. If you would like to order a dual-labeled probe, please use our Dual-Labeled BHQ® Probes quick order form. We also offer other fluorogenic probe and primer formats.
Don’t see the modification you are looking for, or require a larger synthesis scale? We would be happy advise if we can create your specific oligo. Just ask us at techsupport@biosearchtech.com.
A few ordering tips...
Building Oligos with RNA Bases
Indicate that your are inserting an RNA base by selecting rA, rC, rG or rU from the Internal Modification drop down menu or by checking the RNA checkbox if your sequence is entirely RNA.
Building Oligos with Internal Modification(s)
1) If your oligo requires an internal modification, make a selection and then click the ADD button. The internal modification will be inserted at the point of the cursor.
2) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification.
Building Oligos with Wobble/Degenerate Bases (What are Wobbles?)
To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc.
Synthesis Scale vs. Final Yield
The synthesis scale and final yield are not the same due to the several steps involved in probe/primer synthesis. These steps include coupling of each base, cleavage of oligonucleotide from solid support and purification steps. The combination of these steps cause the final yield of the oligo to be less than the synthesis scale (starting material).
Ordering Oligos Delivered in Plates
Ordering a LOT of oligos? Visit our Oligos in Plates webpage where you can download our column or row format excel order forms. Prices start at $0.15 per base with a minimum order of 48 oligos.
