详细描述：
禽甲型流感（H7N9）ValuPanel™试剂*是ValuPanel系列检测试剂的一部分。ValuPanel试剂由检测不同病原菌株遗传信号的探针和引物组成，能够区分在2013年流行的导致禽流感并能够感染人类的禽流感H7N9病毒。这种病毒不同于早期的禽流感病毒株H5N1和仅在鸟类中发现的禽流感病毒株。
该Panel由双标记BHQ探针组成，用于qPCR实验中，以区分H7N9病毒株。这个新的ValuPanel包含以下几种基因的探针和引物：
通用甲型流感病毒
甲型流感病毒亚型H7和N9
人类RNase P（提取对照）
H7和N9亚型的寡核苷酸序列由世界卫生组织（WHO）在中国国家流感中心（CNIC）的流感备案和研究合作中心设计。通用甲型流感和RNase P序列由美国疾病控制和预防中心（CDC**）设计并获得许可。N9亚型的正向和反向引物序列用的是2013年4月15日的修订方案中列出的CNIC-WHO的优化序列。所有探针用FAM和Black Hole Quencher（BHQ）染料标记，且采用HPLC纯化。LGC Biosearch是BHQ染料的发明者，并且是目前在标记探针上使用真正的黑暗猝灭剂BHQ的制造商。
*仅供研究使用。不用于诊断程序。
** CDC不认可产品或服务。
Avian Influenza A (H7N9) ValuPanel™ Reagents* are now part of the ValuPanel line of detection reagents. ValuPanel Reagents are composed of probes and primers that resolve genetic signatures of different pathogen strains. These signatures discriminate the H7N9 virus that causes avian (bird) flu, and capable of infecting humans when circulating in 2013. This virus differs from earlier avian flu strains including H5N1, and others found only in birds.
This panel consists of dual-labeled BHQ® probes for use in real-time qPCR to discriminate H7N9 viral strains. This new ValuPanel contains probes and primers based on genetic signatures for the following:
Universal Influenza A
Influenza A subtypes H7 and N9
Human RNase P (Extraction Control)
Oligonucleotide sequences for the H7 and N9 subtypes were designed by the World Health Organization (WHO) Collaborating Center for Reference and Research on Influenza at the Chinese National Influenza Center (CNIC) in Beijing, China. Universal Influenza A and RNase P sequences were designed by and licensed from the US Centers for Disease Control and Prevention (CDC**). Sequences for the forward and reverse primers for the N9 subtype correspond to the CNIC-WHO’s optimized sequences as listed in the revised protocol dated April 15th, 2013. All probes are labeled with FAM and Black Hole Quencher® (BHQ®) dyes, and are HPLC purified. LGC Biosearch is the inventor of the BHQ dyes and is the sole manufacturer of the true dark quencher currently used for the labeled probes included in the Avian Influenza A (H7N9) panel.
*For Research Use Only. Not for use in diagnostic procedures.
**CDC does not endorse products or services.
