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Scorpions®探针, 单分子, 5' FAM/Internal BHQ-1/HEG

Scorpions® Probe, Uni-molecular, 5' FAM/Internal BHQ-1/HEG

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详细描述：
用于PCR分析的BHQ（Black Hole Quencher，黑洞淬灭剂）标记的Scorpions引物跟双标记探针和分子信标探针不一样，其将引物和探针结合在一个分子里面，引物在3'末端，探针包含在5‘端发夹环结构中。 Scorpions引物利用单分子机制，在实时定量PCR用于瞬时荧光的溶液中能够更快地起作用。与分子信标类似，这些引物在PCR循环期间不需要酶切割探针。这些特点使Scorpions引物成为快速实时定量PCR，终点PCR，SNP检测和基因定量的重要工具。 相比其它PCR探针形式，Scorpion引物具有许多优势： 1. 卓越的特异性 2. 极好的信噪比 3. 容易实现多重 蝎形结构：Scorpions引物是与其它FRET探针不同的探针/引物杂交体，其设计使得它们只有在扩增期间掺入PCR产物时才发光。这些引物结合了两个不同的结构：1）靶特异性DNA探针序列和2）靶特异性PCR引物。 DNA探针序列：DNA探针元件在3'和5'末端具有一段自身互补配对的茎序列，这段茎序列将目标特异性区域维持在中间的发夹状茎环中。荧光基团分子共价连接到5'端，黑暗猝灭剂（即黑洞猝灭剂BHQ染料）连在茎环结构的3'端。荧光基团和BHQ淬灭染料通过茎结构紧密相邻，确保在热循环之前的荧光能够被有效猝灭。 靶特异性引物：靶特异性PCR引物通过一个blocking moiety与DNA探针序列的3'端共价连接。该moiety能够阻止聚合酶延伸到探针元件中，从而避免由此造成的假阳性。 BHQ® (Black Hole Quencher®)-labeled Scorpions primers for PCR analysis are unlike dual-labeled probes and Molecular Beacons because they combine primer and probe in one molecule, with the primer at the 3' end and the probe contained within a hairpin-loop structure at the 5' end. Scorpions primers utilize a uni-molecular mechanism that acts faster in solution for instantaneous fluorescence in real-time PCR. Similar to Molecular Beacons, these primers do not require enzymatic cleavage of the probe during PCR cycling. These qualities make Scorpions primers valuable tools for rapid, real-time PCR, endpoint PCR, SNP detection, and gene quantification. Scorpions primers provide a number of important advantages over other PCR probe formats: Exceptional Specificity Excellent S:N Easily Multiplexed Scorpions Architecture: Scorpions primers are probe/primer hybrids unlike other FRET probes, whose design is such that they emit light only when incorporated into the PCR product during amplification. These primers incorporate two distinct structures: 1) a target-specific DNA probing sequence and 2) a target-specific PCR primer. DNA Probing Sequence: The DNA probing element incorporates a self-complementary stem sequence at the 3' and 5' ends that holds the target specific region in a hairpin loop. A fluorophore molecule is covalently linked to the 5' end and, the dark quencher, a Black Hole Quencher dye, is linked to the 3' end of the loop structure. The fluorophore and BHQ dye are held in close physical proximity by the stem structure to ensure efficient quenching prior to thermal cycling. Target-specific Primer: The target-specific PCR primer is covalently linked to the 3' end of the DNA probing sequence through a blocking moiety. This moiety prevents polymerase extension into the probing element which could manifest as false positives.
https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/scorpions-primers#?tab=product-info
货号 名称 单位 购买
SCP-FB1-2 Scorpions®探针, 单分子, 5' FAM/Internal BHQ-1/HEG 200 nmol 商城订购

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