详细描述：
SuperRox是ROX（羧基-X-罗丹明）荧光基团的特殊配制版本。这种染料通常用作阴性参照来标准化与PCR扩增无关的信号变化。使用ROX阴性参照可控制由加样误差、溶液蒸发、基线荧光不稳定性以及激光或光源异常等导致的孔间不一致性。
SuperROX以更大水溶性标准配置，目的是为了防止“ROX下降”，即在整个PCR反应中出现的ROX信号的线性降低。据推测，这种下降是由于疏水性ROX分子聚集成分子间同源二聚体，伴随着荧光信号的“静态猝灭”导致的。SuperROX是不参与PCR反应的辅助试剂。SuperROX信号在整个实验过程中保持一致，因此提供了稳定的参考以校正基线荧光和归一化放大的信号。
SuperROX仅用于需要阴性参比染料和具有能够分辨ROX荧光基团的光学器件（Abs Max 585nm / Em Max 606nm）的仪器中。SuperROX通常在更终反应混合物中以100-300纳摩尔的浓度使用。在某些仪器上可能需要进一步的浓度优化。
SuperRox is a specially formulated version of the ROX (carboxy-X-rhodamine) fluorophore. This dye is commonly used as passive reference to normalize signal variations not related to the PCR amplification. Irregularities controlled with a ROX passive reference include inconsistency in pipetting, evaporation of solution, instability of baseline fluorescence, and laser or light source anomalies which all produce well-to-well variation.
SuperROX is formulated for maximum water solubility so as to guard against “ROX drop”, a linear decrease in ROX signal occurring throughout the PCR reaction. Presumably, this drop is due to aggregation of hydrophobic ROX molecules into intermolecular homodimers, accompanied by “static quenching” of the fluorescent signal. SuperROX is an ancillary reagent that does not participate in the PCR reaction. The SuperROX signal remains consistent throughout the experiment thus providing a stable reference to correct the baseline fluorescence and normalize the amplified signal.
SuperROX is for use only in machines requiring a passive reference dye and with optics capable of resolving the ROX fluorophore (Abs Max 585 nm / Em Max 606 nm). SuperROX is typically utilized at concentrations between 100 and 300 nanomolar in the final reaction mix. Further concentration optimization may be necessary on certain model instrumentation.
