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分子信标, 33 Bases, 5' Quasar 670/3' BHQ-2

Molecular Beacon, 33 Bases, 5' Quasar 670/3' BHQ-2

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详细描述:
分子信标是一类具有猝灭基团和荧光报道基团的双标记FRET探针。其与传统的双标记探针的区别在于分子信标在3'和5'末端掺入了一段短的(约5 bp)互补的茎序列。 这些互补序列杂交能够形成报告基团和猝灭基团在空间上紧靠在一起的“茎环”结构。这种替代构象使分子信标具有很强的分辨能力,能够识别仅具有单个核苷酸差异的不同目标序列。分子信标另一个不同于传统双标记探针之处在于它们不依赖于由Taq聚合酶的5'外切核酸酶活性引起探针水解产生荧光,因此,PCR后的溶解曲线是可能的。 就像标准双标记BHQ®探针一样,分子信标也进行多重定量。多重定量使用多个光谱分辨的荧光探针能够同时检测几个靶标。 LGC Biosearch分子信标得到了来自新泽西州纽瓦克的公共健康研究所许可进行销售。有关分子信标探针设计信息,请参阅公共健康研究所的分子信标网站。 Molecular Beacons are a type dual-labeled FRET probes incorporating a quencher and a fluorophore reporter molecule. They differ from traditional dual-labeled probes, due to the incorporation of a short (~5 b.p.) complementary stem sequence at the 3' and 5' ends. These complementary sequences hybridize to form a “stem-loop” structure which holds the reporter and quencher close together in space. This alternate conformation makes Molecular Beacons more discriminatory, allowing them to resolve different target sequences differing by a single nucleotide. Molecular Beacons also differ from traditional dual-labeled probes because they do not rely on probe hydrolysis from the 5' exonuclease activity of Taq polymerase to generate its fluorescence, therefore, a post-PCR melt curve is possible. Just like standard Dual-labeled BHQ® probes it is possible to multiplex assays using Molecular Beacons. Multiplexing allows for the detection of several targets simultaneously using multiple spectrally resolved fluorescent probes. Molecular Beacons are sold under license from the Public Health Research Institute, Newark, NJ. For information on designing Molecular Beacons please refer to The Public Health Research Institute's Molecular Beacons website.
https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/molecular-beacons#?tab=product-info
货号 名称 单位 购买
MBO-Q6B2-2sp-33BASES 分子信标, 33 Bases, 5' Quasar 670/3' BHQ-2 20 nmol 商城订购
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